Congratulations to Laszlo Nagy, who has just received a major grant from the Lendület Program of the Hungarian Academy of Science. This program provides support for promising Hungarian researchers who have pursued postdoctoral studies overseas and who wish to return home to set up their own research groups. Laszlo will return in September to the Biological Research Centre, Szeged , where he will establish a group working on fungal evolution and comparative genomics.
Congratulations to Alexis Carlson and Rachael Martin, who successfully defended their Master’s theses on April 30 and May 1!
Alexis performed developmental studies on Lentinus tigrinus, which showed that as little as 15 minutes of light exposure early in primordium development is enough to induce production of a normal pileus (without that, a coralloid fruiting body develops, as illustrated here). Alexis also generated two inbred monokaryons for genome sequencing at the JGI, which opens the door to developmental genetics in my favorite mushroom.
Rachael’s research involved analysis of ITS sequences from basidiomycetous endophytes of rubber trees Hevea spp. It turns out that the most common basidio endophytes are white rot (not brown rot–why?) species in the Polyporales, my favorite order of Agaricomycetes (and the focus of the PolyPEET project). Not all the endophytes are polypores, hwoever. Rachael’s analyses also showed that the bizarre scale insect parasite/mutualist Septobasidium can live as an endophyte—with no scales to be seen. This work was conducted under the supervision of Romina Gazis.
Alexis and Rachael received Bachelor’s degrees at Clark in 2013 and continued on into our Fifth Year Master’s program. Here are some photos from the years:
Genome annotation team: Alexis, Rachael, Albee Ling, Darcy Young and Nathan Kallen ca. 2011.
Alexis, NAMA 2012
Alfredo Justo, Jaya Seelan, Rachael and Alexis, NAMA 2012
Adirondacks 2012, with Rachael and Alexis sixth and eighth from left
Fredo and Alexis at MSA
Rachael and Manfred Binder inoculating the bins at Fisherville
What: “Bot Div” will provide an introduction to the plants and macroalgae of New England, including systematics and natural history. The course will focus on the Hadwen Arboretum, which is just a few blocks from the Clark main campus. The Arboretum was donated to Clark by Obadiah Hadwen (1824-1907) and presents a mix of native and exotic plants, including invasive species. Bot Div students will create a series of “plant portraits” that will contribute to a web-based virtual flora of the Hadwen Arboretum. The class will also include joint workshops with Prof. Elli Crocker and students in ARTS 128 “Drawing: Sense of Place”. For Biology students, the workshops will develop observational and rendering skills; for Art students the workshops will raise awareness about plant structure and botanical illustration. Products of the workshops will be included in the web-based virtual flora and a student gallery exhibition.
When: Mon/Thurs 1:25-2:40 and Thurs 2:50-5:50; with additional weekend field trips.
Prerequisites: Introductory Biology 101/103 and 102.
Here are some of the plants that can be found in the Hadwen Arborteum:
The Hibbett laboratory at Clark University seeks a talented undergraduate with interests in phylogenetic methods for a NSF-supported research experience associated with the Open Tree of Life Project (described below). Candidates should have experience in analytical methods for molecular systematics (sequence database searches, alignment, and phylogenetic analysis). Interest in fungi would be helpful, but is not essential.
“The Open Tree of Life Project seeks to develop tools to enable synthesis of phylogenetic trees and taxonomy into a comprehensive phylogeny of all life that can be updated by members of the systematics community. A key requirement of this project is that the source trees be available in electronic form. However, in a recent survey of fungal (and other) phylogenies, we found that only about 17% of published fungal phylogenetic studies have available trees. To address this situation, and to provide training in phylogenetic methods, we propose to have students supported with REU funds generate phylogenetic trees that correspond to important published studies in fungal systematics for which there are no treefiles available (i.e., following the Materials and Methods sections of published works, students will obtain and analyze data in an effort to regenerate published phylogenetic trees). These analyses will then be published in PLOS Currents Tree of Life, deposited in TreeBASE and Dryad, and integrated into the Open Tree phylogeny database using tools that the project has developed (currently, Phylografter is our tool for tree editing, taxon mapping, and integration, but it may be replaced by summer 2014). This work is important and necessary, because the vast majority of published phylogenies do not have trees available in electronic form, and many researchers who have been contacted have been unable to provide the trees. This work will provide the students with extensive experience in phylogenetic methods, and it will also demonstrate the importance of data archiving to achieve reproducibility in phylogenetic studies.”
Support is available for ten to twelve weeks, beginning as early as June 1, 2014. To apply, please send a single PDF file containing a cover letter (describing relevant experience, and stating dates of availability), resume, and contact information for two references to firstname.lastname@example.org. This position is available for current undergraduates who are U.S. citizens, U.S. nationals, or permanent residents of the United States. Applications will be collected until March 26 (or until the position is filled).
This semester we are fortunate to have three new undergraduate researchers in the lab, Sara Waldman, Dorothy Tang and Christie Joyce. Sam Kovaka is also continuing to work with us.
Sam is continuing his bioinformatics work with us. Presently, he is retrieving photoreceptor genes from the genomes of Lentinus tigrinus and many other fungi. This work will complement experimental studies on light-dependent pileus induction in L. tigrinus that will be performed by Christie and Alicia.
Sara Waldman. The little Petri dishes on the counter contain Fraxinus embryos.
Sara is working with Mitchell Nuhn on the Boletinellus merulioides/Fraxinus americanus symbiosis. She is trying to establish co-cultures of the fungus with the plant and compare survivorship of the plant with and without its fungal partner. The challenge is that the plant embryos have to be dissected out of the seed and germinated on agar to obtain seedlings. Tricky.
Dorothy and Christie are working on our emerging Lentinus tigrinus development projects, along with Alexis Carlson, Alicia Knudson and Laszlo Nagy. Dorothy is helping to obtain and genotype single spore isolates (haploids) that we hope to use in a bulk segregant analysis aimed at understanding the genetic basis of the secotioid phenotype. Christie will be helping develop a system for fruiting L. tigrinus under different wavelengths of light, in an effort to understand the mechanism of pileus induction. Pictures of L. tigrinus in all its forms are posted here.
- Christie Joyce
Dr. Tomoko Wada has just arrived for a six-week visit. Tomoko is a Research Fellow of the Japan Society for the Promotion of Science (JSPS) at the Center for Conservation and Restoration Techniques, of the National Research Institute for Cultural Properties in Tokyo. Her research interests include fungal communities occurring in historical wooden buildings and artworks.
Today our friend Otto Miettinen and his family, Annina and Kelo, returned to Finland. It seems like only yesterday that they arrived.
Otto will complete his Marie Curie Fellowship, and then transition into a new position as curator of fungi in the Botanical Institute in Helsinki. It is always a little sad when people leave the lab, but it is great that Otto will take up this new post, and I trust that we will have opportunities to collaborate and visit in the future. We had a nice going-away party in Otto’s furniture-free apartment. Otto impressed us in so many ways, not least of all for his modern adaptation of the standing writing desk.
NSF has just announced the Genealogy of Life, or “GoLife”, program solicitation (hats off to the NSF Office of Acronyms–we thought AVAToL could not be beat). A note about GoLife has been posted at the Open Tree of Life Project.
Sam Kovaka’s Directed Study (BIOL 299) project last semester involved creation of a large phylogenomic tree using fungal genomes housed at the JGI MycoCosm portal. Sam implemented a pipeline that uses a set of gene clusters from 186 fungal genomes available on MycoCosm in mid-2013. The genes are aligned and concatenated, poorly aligned regions are removed with Gblocks, and the phylogeny is estimated with RAxML. The tree is then parsed into its component clades using least common ancestor statements. The strategy for parsing the tree is similar to that employed in the “mor” project that was developed in my lab way back in 2005, although that used only one gene, nuc-lsu rRNA. To read more about Sam’s project, visit the web site he created, or go straight to the tree below and then click on the terminals to open up the subclades (e.g., Agaricomycotina). I believe that this is the most complete genome-based fungal phylogeny currently available. Thanks to Igor Grigoriev, Bobby Otillar and Robert Riley at JGI for helping Sam (who is a second-year undergraduate in our lab) with this project.
I am just back from a presentation at the Science Café Woo, hosted by the Nu Café in Worcester, and organized by Ana Maldonado-Contreras and Kelly Hallstrom from UMass Medical School (Kelly was a student researcher in my lab at Clark while she was an undergraduate, and worked with my former PhD student, Jason Slot). Tonight, I talked about the work that I did with Dimitris Floudas and many others on the evolution of white rot, and its possible correlation with the decline in coal deposition at the end of the Permo-Carboniferous (and how this result was overblown in the blogosphere). This was one of the most fun, energizing events that I have done in a long time. I have always been invigorated by my interactions with non-professional biologists, whether through the Boston Mycological Club or other organizations, and this was no exception. I certainly enjoy teaching undergraduates at Clark University, but non-professional/non-student audiences really bring something special, namely pure interest and curiosity, unburdened by concerns about grades and career advancement. Tonight’s crowd was engaged and relaxed, and they asked great questions! This experience reminded me why I got into biology in the first place, and it highlights the importance of citizen-science.
This was the eighth Science Cafe this year. The last one was given by my colleague John Baker. Kelly and Ana just received an Outreach Seed Grant from the American Society for Biochemistry and Molecular Biology, so there will be more of these events coming in Worcester in 2014.
Update 12/31/13: Karl Hakkarainen has a piece about the Science Cafe event on Worcester Connects. Karl’s article led me to new examples of how the study with Dimitris et al. has been skewed in the on-line press, including one piece that asks “What caused the great coal crash of 300 million years ago?“